米非司酮对早孕外周血和蜕膜组织自然杀伤细胞亚群的影响

[]1000-4718(2000)02-0146-041,2,1,2(11,510632;21,510632)[]:,NK:NK;E2P4NKCD56+NK,CD56+CD16+CD16+NK:,CD56+NK,[];,;;;[]R392132[]A(progesterone,P4),,,(mifepristone),,(progesteronereceptor,PR)P45,P4PR;P4,,(naturalkiller,NK),[1],,NK,()(earlypregnantdecidua,EPD),14,2227,[]1999-05-10[]1999-10-11Tel020-85220298(241571199),,68,,,,4,6h()(earlypregnantperipheralblood,EPPB)4ml,14()(mifepristone-treateddecidua,MTD),24h()150mg,14,2228,(241552170),,68()(mifepristone-treatedperipheralblood,MTPB),14,4ml,()NK[2](11077,),:CD16-FITC(Immunotech)CD56-PE(DAKO)()(estradiol,E2)P4E2,P4641ChineseJournalofPathophysiology2000,16(2):146149tt,€xsNK1:CD56+CD56+CD16+CD16+(P0101,P0105)Fig1ThepercentageofNKcellularsubcytesindeciduabetweenearlypregnantandmifepristone-treatedgroups3P0.05,33P0.01,vsmifepristone-treatedgroup1NKNKNK,NKCD56+CD16+CD16+;E2P4,11NKTab1ThepercentageofNKcellularsubtypesandinperipheralbloodlymphocytesbetweenearlypregnancy,mifepristone-treatedpregnancy(€xs,%)PhenotypeEPPB(n=14)MTPB(n=11)CD56+CD16-3.940.973.731.09CD56+CD16+9.933.369.012.99CD56-CD16+10.654.889.784.50E2(pmol/L)2033.19424.822121.88960.86P4(nmol/L)21.579.7823.844.99EPPB=Earlypregnantperipheralblood;MTPB=mifepris2tone-treatedperipheralbloodNK2,CD56+,CD56+CD16+CD16+;CD56+CD16+CD16+CD56+;CD56+CD56+CD16+CD16+,CD56+NK,CD56+CD16+CD16+NK2NKTab2ThepercentageofNKcellularsubtypesinperipheralbloodlymphocytesanddecidualcellsofmifepristone-treatedgroup(€xs,%)PhenotypeMTPB(n=11)MTD(n=11)CD56+CD16-3.731.0946.075.57CD56+CD16+9.012.9935.913.563CD56-CD16+9.784.5033.742.0733P0.01,vsCD56+CD16-;MTPB=mifepristone-treat2edperipheralblood;MTD=mifepristone-treateddecidua,(progesterone2inducedblockingfactor,PIBF)[3]TJ6[4],NK[3,4],PRPR,PR(600mg)12hPR[5],PR,P4,P4PR,Fang[6],215mg,E2P4,24hE2P4,,150mg24hE2P4,E2P4,3-,E2741NK,CD56+,CD56+CD16+CD16+NKCD56+[2]E2,CD56+,CD56+CD16+CD16+,CD56+CD56+CD16+CD16+NK,CD56+NKCD56+CD16+CD16+NK,CD56+NKTJ6,TJ6,[7],CD56+NK,NK;,CD56+CD56+CD16+CD16+NKCD56+NKCD16+NK,CD56+NKCD56+CD16+CD16+NKNK,,PR,CD56CD16,NKNK[8],CD56+NK,,Whitelaw[9]CD56+NK,[10](killerinhibitoryreceptors,KIRs),CD56+NKPR,KIRsPR,CD56PRKIR,,,,,PR,PR,CD56+NK,,,[][1]MonterrosoVH,HansenPJ.Regulationofbovineandovinelymphocyteproliferationbyprogesterone:modulationbysteroidreceptorantagonistsandphysiologicalstatus[J].ActaEndocrinol,1993,129:5325351[2],,.[J].,1999,15(7):6196221[3]RaghupathyR.Th1-typeimmunityisincompatiblewithsuccessfulpregnancy[J].ImmunolToday,1997,18:4784821[4],.TJ6:[J]1,1997,16(2):91931[5]ZaytsevaTS,GoncharovaVN,MorozovaMS,etal.TheeffectofRU486onprogesteroneandoestrogenreceptorconcentra2tionsinhumandeciduaonearlypregnancy[J].HumReprod,1993,8(8):128812921[6]FangX,WangS,MitchellBF.EffectsofRU486onestro2gen,progesterone,oxytocin,andtheirreceptorsintheratuterusduringlategestation[J].Endocrinology,1997,138(7):276327681[7]NicholsTC,KangJA,AngkachatchaiV,etal.Expressionofamembraneformofthepregnancy-associatedproteinTJ6onlymphocytes[J].CellImmunol,1994,155(1):2192291[8]GuimondMJ,LurossJA,WangB,etal.Absenceofnatu2ralkillercellsduringmurinepregnancyisassociatedwithre2productivecompromiseinTgE26mice[J].BiolReprod,1997,56:1691791[9]WhitelawPF,CroyBA.Currenttopic:Granulatedlympho2cytesofpregnancy[J].Placenta,1996,17:5335431[10]Rouas-FreissN,GoncalvesRMB,MenierC,etal.Di2rectevidencetosupporttheroleofHLA-Ginprotectingthefetusfrommaternaluterinenaturalkillercytolysis[J].ProcNatlAcadSciUSA,1997,94:11520115251841TheeffectofmifepristoneonnaturalkillersubsetsofperipheralbloodanddeciduainearlypregnancyHOUQian1,2,ZENGYao-ying1,WANGZi-neng2(11InstituteofTissueTransplantationandImmunology,JinanUniversity,Guangzhou510632;21DepartmentofGynaecologyandObstetrics,FirstAffiliatedHospital,JinanUniversity,Guangzhou510632,China)[Abstract]AIM:Toinvestigatetheeffectofmifepristoneonnaturalkiller(NK)subpopulationsofperipheralbloodanddeciduainearlypregnancy.METHODS:FlowcytometrywasusedtodetecttheexpressionofCD56andCD16onlymphocytesofdeciduaandperipheralbloodinearlypregnancy,mifepristone-treatedpregnancy.RE2SULTS:Thepercentagesofdifferentnaturalkillersubsetsinperipheralbloodbetweenearlypregnancyandmifepris2tone-treatedpregnancywerealmostidentical.Theserumlevelsofestradiolandprogesteroneinmifepristone-treatedpregnancywereslightlyhigherthaninearlypregnancy.ThepercentageofdecidualCD56+NKcellinearlypregnancywassignificantlyhigherthaninmifepristone-treatedpregnancy.TheCD56+NKcellswerepredominantlymphocytepopulationofdeciduainmifepristone-treatedpregnancy,butinwhichtheCD56+CD16+andCD16+NKcellsweremajorlymphocytesubpopulationsofperipheralblood.CONCLUSION:Mifepristoneactedprincipallyonfeto-ma2ternalinterface,itblockedtheproliferationanddifferentiationofdecidualCD56+NKcellsandinducedembryoim2munerejection.[MeSH]Decidua;Killercells,natural;Mifepristone;Estrogens;Progesterone941