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4 8环境工程学报Vol.4,No.820108ChineseJournalofEnvironmentalEngineeringAug.2010任宏洋1,2 张代钧2 丛丽影2 张 萍2(1.,610500;2.,,400030) ,,。,,Mann-Ken-dell,。0.2345±0.0115mgCOD/mgNH+4-N。 X703 A 1673-9108(2010)08-1726-03ExperimentalstudyondeterminationofnitrifyingbacteriayieldcoefficientsusingrespirometryRenHongyang1,2 ZhangDaijun2 CongLiying2 ZhangPing2(1.SchoolofChemistryandChemicalEngineering,SouthwestPetroleumUniversity,Chengdu610500,China;2.KeyLaboratoryforExploitationofSouthwestChinaResourcesEnvironmentalDisasterControlEngineering,MinistryofEducation,CollegeofResourcesandEnvironmentalScience,ChongqingUniversity,Chongqing400030,China)Abstract Basedonthecouplingofanabolismandenergymetabolisminnitrification,theelectronstreaminnitrifyingprocesswasanalyzed,andrelationshipbetweenautotrophicnitrifyingbacteriagrowthandoxygenconsuminginammoniumoxidizingprocesswasestablished.Accumulatedoxygenconsumingandoxygenuptakerate(OUR)innitrifyingprocessweremeasuredbyelectrolyticrespirometerinbatchexperiments.WiththemethodofMann-Kendelltrendtest,thetimenitrifyingbacteriagotinendogenousrespirationandtheOURinen-dogenousrespirationweredetermined,andthentheoxygenconsuminginammoniumoxidizingwascalculated.Nitrifyingbacteriayieldcoefficientsinexperimentswasestimated,whichwas0.2345±0.0115mgCOD/mgNH+4-N.Keywords respirometry;nitrifyingbacteria;yieldcoefficients:(50378094);:2009-09-26;:2009-11-05:(1980～),,,,:,。E-mail:renhongyang@swpu.edu.cn ,。,,,[1]。、。[2～4],[5,6]。,。1 [7]。,,NH+4NO-3,NO-2,NH+4+2O2※NO-3+2H++H2O。NH+4,,O2,,CO2,。(fe)(fs)1[8]。(fs)。,NH+4-N、O21。8:1mgNH+4-NNO-3,47mmol,47×fsmmolCO2,。(ThOD),8gCODCO2,1mol,CO247×fsmmol,47×8×fsmgCOD;O247×(1-fs)mmol,47×8×(1-fs)mgO2。,NH+4[5,6]。1gNH+4-N(COD),(1):YA=4/7×8×fs1+iN/COD×4/7×8×fs(1):YA—;iN/COD—;fs—。C5H7O2N,iN/COD=(14mgN)/(160mgCOD)=0.087mgN/mgCOD。1,NH+4-NNH+4-NO2:SNHOUf=1+0.087×4/7×8×fs4/7×(1-fs)×8(2):SNH—NH+4-N;OUf—NH+4-N。(2):fs=(32/7×SNH-OUf)(32/7×SNH+32/7×0.087×OUf)(3)1 Fig.1 Stoichiometriclinksduringnitrifyingprocess2 2.1 O2。2。O2,,,,,,,,O2O2,H2。,,,。O2,6min。2 Fig.2 Schematicdiagramoftheexperimentalset-upSBR,,(NH4)2SO4,NaHCO3,1L0.4mL,ZnSO4·5H2O80mg/L;FeSO4·7H2O100mg/L;CuCl2·2H2O20mg/L;MgCl2·6H2O50mg/L;H3BO320mg/L。pHDOpH,DO2.5～3mg/L,pH7.6～8.0,(30±1)℃。2.2 300mL,3,4h,。(30±1)℃,pH7.6～8.0。1L,,O2,。2.3 pH:pH(PB-10,Sartorius);NH+4-N:;:(BI-2000,BioScience)。3 OUR3,,NH+4-N,OUR,17274NH+4-N,,OUR,。te,0～te,NH+4-N,,te,。NH+4-NOUf:OUf=OU-OURe×te(4):OU—0～te;OURe—。3 OURFig.3 OURandO2consumingprofileinrespirometryte,te,OUR,。Mann-kendallOUR[9]。OUR{OUR1,OUR2,…OURn}[9]:ZMK=(S-1)/n(n-1)(2n+5)/18S00S=0(S+1)/n(n-1)(2n+5)/18S0(5):S=∑n-1i=1∑nj=i+1sgnOURj-OURi;sgn:sgn(θ)=1 θ00 θ=0-1 θ0n10,ZMK。,,α,ZMKU1-α/2,ZMKU1-α/2,,,;ZMKU1-α/2,,。,α0.05,te438min,OUR,。OURe3.011mg/(L·h)。(4)OUf120.199mg,0.2318mgCOD/mgNH+4-N。30.2318、0.22950.2423mgCOD/mgNH+4-N,2.9%。90%,0.2345±0.0115mgCOD/mgNH+4-N。,0.15～0.20mgVSS/mgNH+4-N[10],0.17mgVSS/mgNH+4-N,C5H7O2N,0.24mgCOD/mgNH+4-N,。4 ,,。,,90%,0.2345±0.0115mgCOD/mgNH+4-N。[1],,,..,2005,28(10):128～132[2],..,2004,26(4):253～255[3],,..,2005,25(10):47～49[4],,..,2006,28(7):493～495[5]ChandranK.,SmetsB.F.Estimatingbiomassyieldcoeffi-cientsforautotrophicammoniaandnitriteoxidationfrombatchrespirograms.Wat.Res.,2001,35(13):3153～3156[6]ChandranK.,SmetsB.F.Optimizingexperimentaldesigntoestimateammoniaandnitriteoxidationbiokineticparam-etersfrombatchrespirograms.Wat.Res.,2005,39(20):4969～4978[7],,,..(),2008,31(1):88～92[8]RittmannB.E.,McCartyR.L.,,,.:.:,2004[9],,..,2005,14(5):589～594[10]..:,20021728